cd69 proteintech Search Results


cd69  (Proteintech)
94
Proteintech cd69
Cd69, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd69+proteintech/CD69+Antibody/pmc11494001-68-35-39
Average 94 stars, based on 1 article reviews
cd69 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
anti mouse cd69  (Proteintech)
92
Proteintech anti mouse cd69
( A and B ) M2-Mφs were induced by IL-4/TGF-β (20 ng/ml each), and qPCR analysis was conducted to measure the expression of the M2 gene ( Arg1 , Mrc1 , and TGF- β) expression in M2-Mφs treated with EV GLN− (20 μg/ml) or EV GLN+ (20 μg/ml) for 48 hours ( n = 3; ***P < 0.001, **P < 0.01, ## P < 0.01, ### P < 0.01 versus the M2 group). ( C and D ) qPCR analysis of chemokine gene expression ( Ccl2 and Cxcl2 ) in THP-1 monocytes treated with EV GLN− preparations or EV GLN+ preparations (20 μg/ml) for 24 hours ( n = 3; ***P < 0.001, **P < 0.01 versus the “CON” group). ( E and F ) Chemotaxis evaluation of (E) conditioned culture medium from EV pretreated THP-1 cells and (F) EVs from splenocytes using a Transwell system, and the migrated cells in the lower chamber were counted using FCA ( n = 3; **P < 0.01, *P < 0.05 versus the CON group). ( G and H ) Mouse splenocytes were treated with ConA or ConA plus EV GLN− or EV GLN+ (20 μg/ml) for 72 hours, and the populations of activated CD4 + T cells and (CD3 + CD4 + <t>CD69</t> + ) activated CD8 + T cells (CD3 + CD8 + CD69 + ) were determined by FCA ( n = 3). ( I and J ) Evaluation of immune responses in mice ( n = 5) intravenously injected with EV GLN− or EV GLN+ (30 μg/mouse) for 4 hours and immune cell populations (F4/80 + Mφs, Ly6C + monocytes, and Ly6G + neutrophils) in the spleen were analyzed using FCA ( **P < 0.01, *P < 0.05 versus the CON group).
Anti Mouse Cd69, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd69+proteintech/APC+Anti-mouse+CD69/pmc10866539-281-39-42
Average 92 stars, based on 1 article reviews
anti mouse cd69 - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
coralite594 conjugated igg secondary antibody  (Proteintech)
93
Proteintech coralite594 conjugated igg secondary antibody
( A and B ) M2-Mφs were induced by IL-4/TGF-β (20 ng/ml each), and qPCR analysis was conducted to measure the expression of the M2 gene ( Arg1 , Mrc1 , and TGF- β) expression in M2-Mφs treated with EV GLN− (20 μg/ml) or EV GLN+ (20 μg/ml) for 48 hours ( n = 3; ***P < 0.001, **P < 0.01, ## P < 0.01, ### P < 0.01 versus the M2 group). ( C and D ) qPCR analysis of chemokine gene expression ( Ccl2 and Cxcl2 ) in THP-1 monocytes treated with EV GLN− preparations or EV GLN+ preparations (20 μg/ml) for 24 hours ( n = 3; ***P < 0.001, **P < 0.01 versus the “CON” group). ( E and F ) Chemotaxis evaluation of (E) conditioned culture medium from EV pretreated THP-1 cells and (F) EVs from splenocytes using a Transwell system, and the migrated cells in the lower chamber were counted using FCA ( n = 3; **P < 0.01, *P < 0.05 versus the CON group). ( G and H ) Mouse splenocytes were treated with ConA or ConA plus EV GLN− or EV GLN+ (20 μg/ml) for 72 hours, and the populations of activated CD4 + T cells and (CD3 + CD4 + <t>CD69</t> + ) activated CD8 + T cells (CD3 + CD8 + CD69 + ) were determined by FCA ( n = 3). ( I and J ) Evaluation of immune responses in mice ( n = 5) intravenously injected with EV GLN− or EV GLN+ (30 μg/mouse) for 4 hours and immune cell populations (F4/80 + Mφs, Ly6C + monocytes, and Ly6G + neutrophils) in the spleen were analyzed using FCA ( **P < 0.01, *P < 0.05 versus the CON group).
Coralite594 Conjugated Igg Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd69+proteintech/CoraLite+488-conjugated+Anti-mouse+CD69/pm39694008-98-11-15
Average 93 stars, based on 1 article reviews
coralite594 conjugated igg secondary antibody - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
cd69 pe pe 65105  (Proteintech)
91
Proteintech cd69 pe pe 65105
( A and B ) M2-Mφs were induced by IL-4/TGF-β (20 ng/ml each), and qPCR analysis was conducted to measure the expression of the M2 gene ( Arg1 , Mrc1 , and TGF- β) expression in M2-Mφs treated with EV GLN− (20 μg/ml) or EV GLN+ (20 μg/ml) for 48 hours ( n = 3; ***P < 0.001, **P < 0.01, ## P < 0.01, ### P < 0.01 versus the M2 group). ( C and D ) qPCR analysis of chemokine gene expression ( Ccl2 and Cxcl2 ) in THP-1 monocytes treated with EV GLN− preparations or EV GLN+ preparations (20 μg/ml) for 24 hours ( n = 3; ***P < 0.001, **P < 0.01 versus the “CON” group). ( E and F ) Chemotaxis evaluation of (E) conditioned culture medium from EV pretreated THP-1 cells and (F) EVs from splenocytes using a Transwell system, and the migrated cells in the lower chamber were counted using FCA ( n = 3; **P < 0.01, *P < 0.05 versus the CON group). ( G and H ) Mouse splenocytes were treated with ConA or ConA plus EV GLN− or EV GLN+ (20 μg/ml) for 72 hours, and the populations of activated CD4 + T cells and (CD3 + CD4 + <t>CD69</t> + ) activated CD8 + T cells (CD3 + CD8 + CD69 + ) were determined by FCA ( n = 3). ( I and J ) Evaluation of immune responses in mice ( n = 5) intravenously injected with EV GLN− or EV GLN+ (30 μg/mouse) for 4 hours and immune cell populations (F4/80 + Mφs, Ly6C + monocytes, and Ly6G + neutrophils) in the spleen were analyzed using FCA ( **P < 0.01, *P < 0.05 versus the CON group).
Cd69 Pe Pe 65105, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd69+proteintech/PE+Anti-mouse+CD69/pm37578941-178-0-13
Average 91 stars, based on 1 article reviews
cd69 pe pe 65105 - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier

Image Search Results


( A and B ) M2-Mφs were induced by IL-4/TGF-β (20 ng/ml each), and qPCR analysis was conducted to measure the expression of the M2 gene ( Arg1 , Mrc1 , and TGF- β) expression in M2-Mφs treated with EV GLN− (20 μg/ml) or EV GLN+ (20 μg/ml) for 48 hours ( n = 3; ***P < 0.001, **P < 0.01, ## P < 0.01, ### P < 0.01 versus the M2 group). ( C and D ) qPCR analysis of chemokine gene expression ( Ccl2 and Cxcl2 ) in THP-1 monocytes treated with EV GLN− preparations or EV GLN+ preparations (20 μg/ml) for 24 hours ( n = 3; ***P < 0.001, **P < 0.01 versus the “CON” group). ( E and F ) Chemotaxis evaluation of (E) conditioned culture medium from EV pretreated THP-1 cells and (F) EVs from splenocytes using a Transwell system, and the migrated cells in the lower chamber were counted using FCA ( n = 3; **P < 0.01, *P < 0.05 versus the CON group). ( G and H ) Mouse splenocytes were treated with ConA or ConA plus EV GLN− or EV GLN+ (20 μg/ml) for 72 hours, and the populations of activated CD4 + T cells and (CD3 + CD4 + CD69 + ) activated CD8 + T cells (CD3 + CD8 + CD69 + ) were determined by FCA ( n = 3). ( I and J ) Evaluation of immune responses in mice ( n = 5) intravenously injected with EV GLN− or EV GLN+ (30 μg/mouse) for 4 hours and immune cell populations (F4/80 + Mφs, Ly6C + monocytes, and Ly6G + neutrophils) in the spleen were analyzed using FCA ( **P < 0.01, *P < 0.05 versus the CON group).

Journal: Science Advances

Article Title: Nutrient availability regulates the secretion and function of immune cell–derived extracellular vesicles through metabolic rewiring

doi: 10.1126/sciadv.adj1290

Figure Lengend Snippet: ( A and B ) M2-Mφs were induced by IL-4/TGF-β (20 ng/ml each), and qPCR analysis was conducted to measure the expression of the M2 gene ( Arg1 , Mrc1 , and TGF- β) expression in M2-Mφs treated with EV GLN− (20 μg/ml) or EV GLN+ (20 μg/ml) for 48 hours ( n = 3; ***P < 0.001, **P < 0.01, ## P < 0.01, ### P < 0.01 versus the M2 group). ( C and D ) qPCR analysis of chemokine gene expression ( Ccl2 and Cxcl2 ) in THP-1 monocytes treated with EV GLN− preparations or EV GLN+ preparations (20 μg/ml) for 24 hours ( n = 3; ***P < 0.001, **P < 0.01 versus the “CON” group). ( E and F ) Chemotaxis evaluation of (E) conditioned culture medium from EV pretreated THP-1 cells and (F) EVs from splenocytes using a Transwell system, and the migrated cells in the lower chamber were counted using FCA ( n = 3; **P < 0.01, *P < 0.05 versus the CON group). ( G and H ) Mouse splenocytes were treated with ConA or ConA plus EV GLN− or EV GLN+ (20 μg/ml) for 72 hours, and the populations of activated CD4 + T cells and (CD3 + CD4 + CD69 + ) activated CD8 + T cells (CD3 + CD8 + CD69 + ) were determined by FCA ( n = 3). ( I and J ) Evaluation of immune responses in mice ( n = 5) intravenously injected with EV GLN− or EV GLN+ (30 μg/mouse) for 4 hours and immune cell populations (F4/80 + Mφs, Ly6C + monocytes, and Ly6G + neutrophils) in the spleen were analyzed using FCA ( **P < 0.01, *P < 0.05 versus the CON group).

Article Snippet: After treatment, cells from each group were collected and stained with fluorescein isothiocyanate (FITC)–conjugated anti-mouse CD3e (553061, BD, Brea, CA, USA, USA), peridinin chlorophyll protein (PerCP)/Cyanine5.5-conjugated anti-mouse CD4 (100434, BioLegend), phycoerythrin (PE)–Cy7–conjugated anti-mouse CD8a (552887, BD), and allophycocyanin (APC)–conjugated anti-mouse CD69 (APC-65105, Proteintech, Wuhan, China) for 30 min. After washing with PBS, the stained cells were analyzed using a flow cytometer (LSRFortessa, BD Biosciences).

Techniques: Expressing, Gene Expression, Chemotaxis Assay, Injection